An .iso file downloaded from the Internet (thus with a Zone.Identifier ADS) opened in Windows 10 will not propagate this “mark-of-the-web” to the contained files.
Here is an example with file demo.iso, marked as downloaded from the Internet:
When this file is opened (double-clicked), it is mounted as a drive (E: in this example), and we see the content (a Word document: demo.docx):
This file is not marked as downloaded from the Internet:
Word does not open it in Protected View:
Didier Stevens 